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KMID : 1024420110150020182
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Food Engineering Progress
2011 Volume.15 No. 2 p.182 ~ p.187
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Protease Activity of Lactic Acid Bacteria Isolated from Korean Traditional Fermented Food
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Kook Moo-Chang
Cho Seok-Cheol
Park Hoon
Kim Seung-Seop
Pyun Yu-Ryang
Choi Woon-Yong
Lee Hyeon-Yong
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Abstract
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A proteolytic lactic acid bacterium was isolated from Korean traditional fermented foods. The isolate BV-26, which had a protease activity (24 U/mg-crude protein), was identified as Lactobacillus plantarum by the API 50CHL kit and 16S rDNA analysis (99.9% of homology), and named as L. plantarum BV-26. Cell growth and protease activity of L. plantarum BV-26 was determined in MRS broth using 5L jar fermentor at 30oC. The maximum growth of L. plantarum BV-26 was reached at 18 hr in MRS broth, while protease activity of BV-26 was detectable at 12 hr and the highest activity was obtained after 16 hr cultivation. Therefore, we expect that the proteolytic lactic acid bacteria, L. plantarum BV-26, may be used as a starter for the fermentation of animal feed. Especially, the fermentation of soybean meal with the strain can be applied for improving feed utilization.
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KEYWORD
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protease, animal feed, lactic acid bacteria, Lactobacillus plantarum
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